Skip to search form Skip to main content You are currently offline. Some features of the site may not work correctly. Somatic hybridization is an important tool for supplying new varieties to be used directly as cultivar genetic improvement programs. In citrus, this technology has been extensively used and has many important implipications.
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Irfan Suliansyah. Protoplasts can be induced to fuse to produce a hybrid plant, which cannot be produced by conventional plant breeding due to incompatibility.
Isolated protoplast are capable of ingesting "foreign" material into the cytoplasm. This material includes the introduction of nuclei, chloroplasts, mitochondria, DNA, plasmids, bacteria and viruses. Protoplasts can be studied as single cell systems. Protoplast can be used to study. Fusi Protoplas. Sitoplasma tanpa inti. Karioplas tanpa sitoplasma.
These so-called cytoplasts contain. In this way one can, for example, transfer CMS cytoplasmic male sterility into another plant. Companies involved in such work have documented the changes in mitochondrial DNA resulting from these procedures in detail and have patented the relevant techniques. FiBL Mengatasi keterbatasan hibridisasi seksual. Tanaman yang tidak berbunga. Tanaman steril jantan.
Tanaman yang inkompatibilitas. Introgresi gen sitoplasma mt-DNA. Perkembangan hirida tanaman melalui fusi protoplas somatik dari. Advantages of somatic hybridization. Limitations of Somatic hybridization. Metode Mekanis. Pemotongan dinding sel dengan pisau. Koleksi protoplasma. Cut plasmolyzed tissue and subsequent deplasmolysis results in expansion and release of the protoplasts from the cut ends of the cell.
In practice this technique is difficult and the yield of viable protoplasts is meager. One advantage, however, is that the deleterious effects of the wall-degrading enzymes on the metabolism of the protoplasts are eliminated. Enzymatic Method. Protoplasm released. Release of isolated cells. Use of enzymes results in a high yield of uniform protoplasts after removal of cellular debris. Protoplasts can originate from different sources:. Spontaneous Fusion.
Induced Fusion. Identification and Selection of somatic. Hybrid Selection. Several markers are used. Culture of the hybrid cells. Hybrid cells are cultured on suitable medium provided with.
Regeneration of hybrid plants. Tahapan Fusi Protoplas. Protoplast Isolation and Culture. Learn more about Scribd Membership Home. Much more than documents. Discover everything Scribd has to offer, including books and audiobooks from major publishers. Start Free Trial Cancel anytime. Uploaded by Annisa Fara Dilla.
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Cytoplast fusion is. Regenerasi tanaman hibrida. Isolasi Protoplas. Pemisahan protoplas dari jaringan tanaman. Metode mekanis. Metode Enzimatik. Plasmolisis Sel. Jaringan Tanaman. Observasi Mikroskopis Sel. Melepas protoplasma. Leaf sterlization, removal of. Protoplast Fusion. Fusion of protoplasts of two different genomes. Anonymous HXLczq3. Rahma Twin. Ethel Lee. Dhanupriya Suresh Kumar. Irfan Khan. Ty Neal.
Hemanth Kumar Ramachandran. Zubair Khalid. O me khan. Saeed Abdulhadi. More From Annisa Fara Dilla. Annisa Fara Dilla. Popular in Hybrid Biology. Joanne Poyourow. Khondaker Riyadh. Resita Reiita. Harish Kakrani. Jhosue Yepez. Siva Srinivas. Mari Perez. Jogendra Singh. Ioana Tamasiu. Carlos Castinov. Ardie Cabatin. Tanmay Kanwar. Genotypes at Bako and Uke, Western Oromia. Premier Publishers.
5843_4. Fusi Protoplas
Irfan Suliansyah. Protoplasts can be induced to fuse to produce a hybrid plant, which cannot be produced by conventional plant breeding due to incompatibility. Isolated protoplast are capable of ingesting "foreign" material into the cytoplasm. This material includes the introduction of nuclei, chloroplasts, mitochondria, DNA, plasmids, bacteria and viruses. Protoplasts can be studied as single cell systems. Protoplast can be used to study.
Somatic fusion , also called protoplast fusion , is a type of genetic modification in plants by which two distinct species of plants are fused together to form a new hybrid plant with the characteristics of both, a somatic hybrid. Uses of somatic fusion include making potato plants resistant to potato leaf roll disease. The resulting hybrid has the chromosomes of both plants and is thus similar to polyploid plants. Somatic hybridization was first introduced by Carlson et al. The somatic fusion process occurs in four steps: . The procedure for seed plants describe above, fusion of moss protoplasts can be initiated without electric shock but by the use of polyethylene glycol PEG.